What it Takes to be a Working Mother essays

What it Takes to be a Working Mother essays I would be confident to say that everyone knows a working mother or two personally. Working mothers are quite the norm and touch every aspect of our modern day society. I am one of the many working mothers in the full time work force today. Personally, I would laugh at the term normal when used in the same sentence as mother. At times my life and is quite the opposite of what others would consider to be normal. I have a full time job and a full time family. I can say that life can be hectic, challenging and rewarding all in a single day at any given time. Each day is filled with the challenge of holding down a full time career and the grace of being a loving mother of challenging children. In the end, the rewards are endless and my goal is clearly to be the best at all I do in my career and for my family. I can personally say that having a full time career and being a single mother is draining for a lack of a better term. I raised my son Alex as a single mother since he was 7 years old. During that time I worked full time as an Escrow Officer in the real estate industry and attended a career college part time. My day started at 5 am with making lunches, checking homework and getting my son off to school. If there was any time left I then felt privileged to enjoy a cup of coffee or do my own hair before rushing out the door. My morning challenge began by taking my son to school, fighting traffic and trying to make a goal of getting it to work on time. My day then continued with the usual rat race of phones, clients and time crunches to the very end. During the day I had to also find time to run down the daily list of personal mental chores in my head. Is my son having a good day and are his grades doing well? Are my finances in order? Do we have milk at home? These were just a few of the normal laundry list of things dancing around in my head daily. I also felt that I wasnt just working a job, but I was working on a career to ...

How to Write a Career-Winning or Award-Winning Executive Resume

How to Write a Career-Winning or Award-Winning Executive Resume As the owner of an executive resume writing company, I am extremely proud to have four writers on my team, including myself, who have won coveted TORI (Toast of the Resume Industry) awards for their Executive Resume, Finance Resume, International Resume, Sales Resume, and New Graduate Resume entries. On February 9th, Laura DeCarlo, President of Career Directors International, author of Resumes for Dummies, and my personal resume writing and business mentor, presented a teleseminar on how to win these competitive awards. While her presentation was targeted toward resume writers and executive resume writers who wish to compete for TORI awards, some of the points she raised apply to any executive or job seeker creating a career-winning resume. Here are some of the points to keep in mind if you want to write a resume or executive resume that rivals the TORI winners from this past year: 1. Create a compelling format. First of all, know your industry and adjust accordingly. For instance, you can take more creative license as a marketing or sales executive than you can as an insurance or finance executive. Once you determine your industry’s comfort level with design, as well as your own, create something that â€Å"pops† while not going overboard. You don’t need fancy graphics programs to design a great looking resume. You might be surprised by how much you can do with Word! For instance, use edge-to-edge design, different backgrounds (with discretion), color saturation variations, etc. Include a little smart art if appropriate – it’s all in Word – or create a chart in Excel and paste that into your resume. Include plenty of white space, as text-dense resumes are not well-received. Print it out before sending – and run it by some colleagues in your industry for their opinion. 2. Watch your language! Use smart word choices, dynamic and varied verbs, and good sentence structure. No misspellings or run-on sentences please! Many hiring managers will dismiss a resume out of hand for a single grammatical error. And if they start getting bored because you started every bullet with the verb â€Å"led† or â€Å"managed,† you could lose them fast. 3. Deliver â€Å"power and punch.† Keep the reader engaged. Pack your executive resume with measurable achievements – metrics and concrete/tangible results. Also, deliver a clear description of the scope of your responsibilities. Share your CAR (Challenge/Action/Result), PAR (Problem/Action/Result) or STAR (Situation/Task/Action/Result) stories that show how you have tackle challenges and what results you have created. These accomplishments will demonstrate what you’re capable of creating for your next company. 4. Convey your Unique Selling Proposition (USP). Tell us what makes you stand out as opposed to anyone else applying for this position. Do this in the first few lines of your resume! Don’t be scared of selling yourself by dropping names and numbers directly into your resume summary. Make yourself shine! 5. Put yourself in the employee’s shoes. Imagine yourself reading your resume as your future employer. What would you be looking for? Would you hire you? As someone reading a resume, you would of course want to see some of the keywords that are essential to the position. That’s just the basics. Once that threshold is past, is this resume enjoyable to read? Do you have to squint to read it? Are you bored? Do you really get who this person is and the difference they could make for your company? Be rigorous in asking – and answering –   these questions. 6. Do your homework. As the time to write your new resume approaches, start looking for formats and content that you like. When you come across something that impresses you, put it in a file on your computer. You can use this file whether you create your own resume or hire someone to do it. At The Essay Expert, we will always be happy to create the type of format you like. I believe any good resume writing company will do that, while steering you gently in the right direction. If you’re a do-it-yourselfer, get some good books on resume writing or executive resume writing. I recommend my e-books, How to Write a WINNING Resume and How to Write a STELLAR Executive Resume, as well as Laura DeCarlo’s Resumes for Dummies. Overall, your resume or executive resume requires high-level storytelling that knocks the reader’s socks off with both an appealing format and impressive language. I can’t tell you how many of my clients come back to me and tell me they’ve received feedback from employers that â€Å"this is the best resume [they’ve] ever seen.† That means you have done something no one has ever done before. That’s what’s required to win a TORI award, and that’s what can get you your dream job. Want to view this year’s TORI Award-Winning masterpieces? Click here. TORI Award Categories are as follows: Best Accounting Finance Resume Best Executive Resume Best Healthcare/Medical Resume Best Hospitality Resume Best Information Technology Resume Best International Resume Best New Graduate Resume Best Sales Resume Save

What factors determines use of a certain device Research Proposal

What factors determines use of a certain device - Research Proposal Example The independent variable will be measured using the coefficient associated with regression of the objector data to be measured. According to Marshall, Data will be collected using mixed methods approach. Data collection tools will include: Key informant interviews, observation and questionnaires. An example of the questions that will be asked involves, According to Ingersoll, Data will be analyzed using contingent tables. The application of the chi-square statistic is used to assess the relationship between the two variables. The investigator takes the observed frequency (O) and compares it to the expected frequency (E) and combined using the following formula. The importance of this study is to understand the factors that determine the use of a device. The results of this study will confirm the already existing data, add to that data or provide new knowledge in the field. This will promote more research in the field and the public and the government will be more interested to know the findings. The plan for this project is as follows: presentation of the research topic, preparation and presentation of the proposal, data collection, data analysis, documentation and final presentation of the

Population Growth and Its Effect on Global Warming Research Paper

Population Growth and Its Effect on Global Warming - Research Paper Example , this brief essay will attempt to briefly analyze this monolithic problem in terms of the exponential growth in human population that has been evidenced over the past 200 years. Furthermore, a determination will be sought to be made with regards to the question of whether human action or natural causes best explain the climate fluctuations that planet earth has recently been chronicling. Lastly, as a function of the previous points of discussion and analysis, the author will attempt to proscribe a reasonable and performable set of steps and solutions which both the scientific community and the world at large could and should seek to employ as a function of ameliorating the stress on planet earth and the issues at hand. Furthermore, as a means of bringing such an understanding about, the analysis will also rely upon relevant scientific publications on the topic as well as verifiable statistics and charts concerning overall levels of climate change and corresponding increases in CO2 e missions by humans over the past several decades. However, noting the global climate change is an issue is not, in and of itself sufficient. As such, the analysis will also consider the rapidly increasing demand that fossil fuels have been projected to assume over the next several decades and posit a potential alternative to further environmental degredation at the hands of seemingly ever expanding human populations. Firstly, there is the point of view that strongly believes that the swings in climate change are the direct result of the presence of high amounts of human CO2 in the atmosphere that is causing a greenhouse effect on the planet and thereby causing world temperatures to rise. Prima fascia of this argument is the belief that human CO2 emissions are responsible for the changes to the global climate. It follows therefore that those which ascribe to this point of view are the most vehement that drastic and immediate changes to the manner in which human beings interact

Assignment Example | Topics and Well Written Essays - 1250 words - 4

Assignment Example It also makes them aware of the expectations and challenges that they are likely to meet in a particular job environment and guide them how to tackle the difficult situations on their own. All this has made this field essential for the students and organizations. In this study, the researcher has observed that a positive application of career counseling is in the rehabilitation of the ex-felons. Ex-felons or ex-criminals are the people who are responsible for some murder, theft, fraud or such crimes in the past, and having completed their sentenced period they want to move in the right lawful directions. However, it is noted that, these ex-offenders face problems in re-entering the society, in finding jobs and even in getting settled somewhere easily. The society does not accept them, the employers do not trust them and people do not want to see them around (Patton, & McMahon, 2006). As a result they not only face psychological complications due to frustration and depression, but the y are also forced to cope with the financial problems by themselves. To help such people, halfway houses are established that not only provide them with monitored shelter and support, but also therapy and guidance, including career counseling. These residences act as 24 hours treatment lodgings that keep a complete record about the deeds of each individual, and keep an eye on each of them for further development. The researcher believes that the role of a career counselor in a halfway house is really challenging. He not only has to guide the persons for the right choice of the career, but he will have to assist them in achieving trust of their employers and in maintaining righteous behavior. Therefore the researcher is going to plan a counseling course for the ex-felons at a halfway house. OBJECTIVES The objective of this project is to map out such a course to career counsel the ex-felons at halfway houses that: 1. Is practical and applicable 2. Can ensure that the ex-law-breakers f eel obliged to continue on their righteous path after the sentence and get settled for a normal life 3. Provides the individuals in question with sense of security and satisfaction in their working environments For this purpose the researcher has selected a halfway house that is accommodating 23 ex-cons (all males) whose crimes range from illegal merchandise and fraudulent to murder in anger. LITERATURE REVIEW Career counseling revolves around three basic variables: Work, Worker and Working Environment (Chappell, Di, & Labour, 2000). The disturbance in any of these may cause imbalance in the whole work plan. Therefore the researcher has based his project around these three essential variables. The individual or worker is quite important as he proves to be an important factor in the failure of a particular working deal. According to Frank Parsons’ tripartite model (2008), the worker should know and understand his own self (his aptitude and potential), job requirement, and then he should select a particular career logically. Therefore, the researcher has made it his first priority to counsel the persons about their wants in accordance to their needs. These needs do not include only the basic needs of food, shelter and security, rather he has suggested his personal inclination too, so that to make work an attractive and interesting to do. The interaction between the worker and his working environment also triggers specific behaviors that lead to progress or destruction.

Techniques For Invitro Pharmacology Lab Report Biology Essay

Techniques For Invitro Pharmacology Lab Report Biology Essay Schild plot: Schild plot is defined as pharmacological method of receptor classification. By using schild plot dose-effect curve for an agonist is determined in the presence of various concentrations of a competitive antagonist for its receptor in the presence of agonist i.e. equilibrium dissociation constant is calculated. The experiment is carried out for series of dose ratios for a given effect. For example the ratio of the dose of agonist (A) to produce a specific effect (e.g.,  half maximal effect) in the presence of the antagonist (B) to the dose required in the absence of the antagonist (A) is calculated. This is determined for several doses of antagonist and then log ((A/A) -1) versus the negative log B is plotted.   If the regression of log ((A/A) -1) on -log B is linear with a slope of -1, then this indicates that the antagonism is competitive and by definition the agonist and antagonist act at the same recognition sites. If the slope of the regression is not -1, then b y definition the antagonist is not competitive or some other condition is in effect. This might include multiple binding sites or pharmacokinetic interactions. Agonist: Agonist is a drug which has both affinity and efficacy. Antagonist: Antagonist is a drug which has affinity and zero efficacy. Affinity:  Affinity is a property of a drug; it measures how tight a drug binds to a receptor. To bind to a receptor a functional group of the drug should bind to the complementary receptor. The binding capacity of the drug defines the action of the drug. Efficacy: Efficacy of a drug can be defined as ability of drug which activates the receptor to produce desired effect after binding. Affinity and efficacy are explained in the equation as: K+1 ÃŽ ± A + R AR* Response K-1 ÃŽ ² K+1 B + R BR No Response K-1 Where A is agonist, B is antagonist, K+1 is association rate constant for binding, K-1is dissociation rate constant for binding ÃŽ ±- Association rate constant for activation ÃŽ ²- Dissociation rate constant for activation By using law of mass action affinity is explained as B + R BR Drug free receptor drug-receptor complex At equilibrium KB = [R] [B] KB = Equilibrium dissociation constant [BR] Hill-Langmuir equation: this equation explains drug occupancy [RT] = [R] + [BR] If [RT] = Total number of receptors then by substituting this in law of mass action equation [RB] = [B] [RT] KB + [B] By this equation it is determined that drug occupancy (affinity) depends on drug concentration and equilibrium dissociation constant. Equilibrium dissosciation constant: EQUILIBRIUM DISSOCIATION CONSTANT (Kd) : It is the characteristic property of the drug and the receptors. It is defined as the concentration of the drug required to occupy 50 % of the receptors. The higher the affinity of the drug for the receptors lower is the Kd value. Mathematically Kd is k2/k1 where k2 is the rate of dissociation of the drug from the receptor and k1 is the rate of association of the drug for the receptor. Receptor (R) and Drug (D) interact in a reversible manner to form a drug-receptor (RD) complex.   Ã‚  Ã‚  Ã‚  Ã‚  Where R =   Receptor   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  D =   Drug (L for ligand is sometimes used in these equations)   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  k1 = the association rate constant and has the units of M-1min-1   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  k2 = the dissociation rate constant and has the units of min-1.     Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  k2 is sometimes written as k-1. If an agonist binds to the receptor, then the interaction of the agonist (D) and the receptor (R) results in a conformational change in the receptor leading to a response. If an antagonist binds to the receptor, then the interaction of antagonist (D) and receptor (R) does not result in the appropriate conformation change in the receptor and a response does not occur. For drugs that follow the law of simple mass action the rate of formation of the complex can be defined by the following equation d[RD]/dt refers to the change in the concentration of [RD] with time (t). Note: the square brackets refer to concentration. This equation indicates that the rate at which the drug receptor complex (RD) is formed is proportional to the concentration of both free receptor (R) and free drug (D). The proportionality constant is k1. The rate of dissociation can be defined by the following equation -d[RD]/dt is the decrease in drug-receptor complex with time This equation indicates that the rate at which the drug-receptor complex (RD) dissociates back to free drug and free receptor is proportional to the concentration of the drug receptor complex. The proportionality constant is k2. When the drug and the receptor are initially mixed together, the amount of drug-receptor complex formed will exceed the dissociation of the drug-receptor complex. If the reaction is allowed to go for a long enough, the amount of drug-receptor complex formed per unit time will be equal to the number of dissociations of drug-receptor complex per unit of time, and the system will be at equilibrium. That is equilibrium has occurred.    Equilibrium can be defined as or k1[R][D] = k2[RD] This equation can be rearranged to give By definition Kd is the dissociation equilibrium constant. Kd has units of concentration as shown in the following equation. Simple competitive antagonism: simple competitive antagonism is the most important type of the antagonism. In this type of antagonism the antagonist will compete with available agonist for same receptor site. Sufficient antagonist will displace agonist resulting in lower frequency of receptor activation. Presence of antagonist shifts agonist log dose response curve to right. A schild plot for a competitive antagonist will have a slope equal to 1 and the X-intercept and Y-intercept will each equal the  dissociation constant  of the antagonist. This can be explained in equation as: Occupancy for agonist [RA] = [A] OR [A]/ KA [RT] KA+ [A] [A]/ KA +1 In presence of competitive antagonist (B) [RA] = [A]/ KA [RT] [A]/ KA + [B]/ KB + 1 Occupancy reduced according to [B] and KB To obtain same occupancy, must increase [A] to [A`] r = [A] / [A] = [B] / [B] Schild equation: r = [B] / KB +1 Where r depends on [B] and KB Applying log on both sides log (r-1) = log[B] log KB Aim: The main aim of the experiment is to measure the equilibrium dissociation constant (KB) for atropine at acetylcholine muscuranic receptors and to determine the drug receptor interactions. Objectives The main objectives of the experiment are as follows To measure the equilibrium dissociation constant for atropine at acetylcholine muscuranic receptors To demonstrate the reversible competitive antagonism of atropine at acetylcholine muscuranic receptors To determine the equilibrium dissociation constant (KB) for atropine at acetylcholine muscuranic receptors by using schild plot. Method Isolation and mounting of Guinea-pig ileum in organ bath Guinea-pig was first sacrificed and then the ileum was collected and transferred into physiological salt solution maintained at 370C. The food particles present in the ileum was expelled out through running Krebs solution through the lumen. Then tissue was tied with a thread at both the ends where one was tied to the mounting hook and the other was attached to the transducer. Preparation of serial dilutions of drug The drugs used in the experiment were acetylcholine (Ach) and atropine. To determine the simple competitive antagonism of atropine at Ach muscuranic receptors serial dilutions of Ach were carried out. Ach was given as 110-2M and from the above concentration of the drug the following concentrations were prepared to the organ bath concentration such as 110-6M, 310-6M, 110-7M, 310-7M, 110-8M, 310-8M, 110-9M and 310-9M Ach. Then atropine was diluted to 110-8M (organ bath) from the given 110-2M concentration. Determination of Organ bath concentration The volume of physiological salt solution (pss) was 20 ml, and each time the volume of drug introduced into organ bath was 20 µl.Therefore if 20 µl of 110-2M drug was introduced into the organ bath then it gives 110-5M organ bath concentration. Mathematical calculation of organ bath concentration: In organ bath we have 20ml of pss which is equal to 20103  µl of pss, if 20  µl of 110-2 M Ach was introduced then the organ bath concentration 20 µl→XM 20ml→10-2M = 20  µl x 10-2 M 20x 103  µl = 110-5M (organ bath concentration). The isolated guinea- pig ileum was mounted onto the organ bath and set up for recording isometric tension of the tissue using chart software in a Mac book. Step-1 Calibration of the experimental apparatus: The chart 5 software was calibrated and the sampling rate was adjusted to 10 samples per second with a maximum input voltage to 10 mV. The baseline was set to zero and then trace was started from the baseline zero then the force transducer was calibrated by placing 1 gram weight and after the calibration the trace produced was stopped for the moment to convert the units of tension into grams by selecting the trace produced previously. Step-2 Sensitisation of preparation: To check the viability of the tissue a response of suitable height was obtained by adding a little high concentration of the drug. Here in the experiment an appreciable recording was noted at 110-7M Ach. Step-3 The time cycle followed to construct a concentration- response curve was 0 seconds to add the drug concentrations 30 seconds to empty the organ bath and refill with fresh physiological salt solution 180 seconds next drug concentration was added to the organ bath. Concentration Response Curve: By making use of the above drug concentrations a concentration response curve was constructed according to the provided time cycle. 20  µl of 110-9M Ach was added into the organ bath at zero seconds at is allowed to stand for 30 seconds, then after 30 seconds the organ bath was emptied and refilled with pss. Pss was allowed to stand for 180 seconds. During the wash period if the peak does not return to the base then it was washed twice or thrice to make sure that all the drug dissociates from the receptors before the next addition of the other drug concentration. Each concentration was repeated twice or thrice until the two consecutive responses were reported with the same peak height. By following the procedure and time cycle, the concentration response curve was constructed with different concentrations of acetyl choline such as 110-9M,310-9M, 110-8M, 310-8M, 110-7M, 310-7M, 110-6M and 310-6M Ach (organ bath concentration). Step-4 Equilibration of Acetylcholine receptors with acetylcholine After step-2 the preparation was washed several times until the peak returned to the base line. Then atropine (110-8M organ bath concentration) was added to the preparation and then set aside for 40 minutes to allow atropine to equilibrate with acetylcholine muscuranic receptors. Step-5 Concentration response curve in the presence of atropine The concentration response curve with acetylcholine was repeated again in the presence of atropine by following the time cycle and procedure, which was same as same step 2.Therefore in step 3 with each addition of acetylcholine concentration atropine was added simultaneously. Step-6 Analysis: The graph pad prism in the Mac book was used to plot concentration response curves in the absence and presence of atropine. Log concentration (acetylcholine) Vs response in grams From the above plot EC 50 values of acetylcholine in the presence and absence of atropine were obtained. Then the distance between the two curves control and response for the atropine presence was denoted by r, where r was called as shift. The shift was calculated mathematically as r= EC 50 of response in the presence of atropine EC 50 of Ach in the absence of atropine From the value of the shift, schild plot was plotted as log concentration of atropine presence against log(r-1). From the schild plot the dissociation constant KB for atropine at acetylcholine muscuranic receptors was determined. Results: As explained above in the procedure serial dilutions of acetylcholine was added to the organ bath, where Ach has produced concentration dependent contractions of the guinea pig ileum as shown in the fig 1. Figure: 1 Trace showing contractions produced by serial dilutions of acetylcholine at muscuranic receptors. As shown in Figure 1 the serial dilutions of acetylcholine are added into the organ bath from 110-7M to 310-6M Ach. Here in the trace it was clearly shown that contractions produced by the acetylcholine have been increased with respect to the concentrations. In step-2 the preparation was washed and added with 110-8M atropine and set aside for 40 minutes to equilibrate the acetylcholine receptors. Figure: 2 Trace showing contractions produced by serial dilutions of acetylcholine at muscuranic receptors in the presence of atropine. In the trace it is clearly shown that, the contractions produced by serial dilutions of Ach from 110-8M to 310-4M in the presence of 110-8M atropine. When Trace 1 and Trace 2 are compared it is evident that the contractions produced by Ach alone (trace 1) were greater than the contractions produced Ach in the presence of atropine (trace 2) which proves the simple competitive antagonism by atropine at muscuranic receptors. A graph is plotted to the log concentration response curve produced by Ach alone against Ach in the presence of atropine. (graph is attatched to the report) From the graph it is known that with the increase in the concentration of Ach, response have been increased when compared to Ach in the presence of atropine and also there is a shift towards right which shows the simple competitive antagonism produced by atropine. From the results produced by Ach alone against Ach in the presence of atropine the fractional difference which is called as shift can be obtained as follows Mathematical Calculation shift r = EC50 of response after atropine (or) in the presence of atropine EC50 of control (or) Ach in the absence of atropine = 2.5110-6 = 8.36 3.0 x10-7 r-1 =8.36 -1=7.36 log(r-1)=log (7.36) =0.86 Partial dissociation constant (PKB) or PA2 is measured to confirm the simple competitive antagonism, where pKB values play an important role in classifying receptors. Therefore PKB =log(r-1) -log [atropine] =0.86 -log (110-8) =0.86 (-8) =0.86+ 8 =8.86 From the above results log EC50 values for control (Ach alone) and Ach in the presence of atropine were given as 3.0e-007 and 2.51e-006 respectively. This shows the molar concentration of Ach which produces 50% of the maximal possible response is higher than the molar concentration response produced by Ach in the presence of atropine. Figure 5: (Graph2) Schild plot If the antagonist is competitive, the dose ratio equals one plus the ratio of the concentration of antagonist divided by its Kd for the receptor. (The dissociation constant of the antagonist is sometimes called Kb and sometimes called Kd) A simple rearrangement gives: Here we have plotted a graph with log (antagonist) on the X-axis and log (dose ratio -1) on the Y-axis. If the antagonist has shown simple competitive antagonism then the slope should be 1.0, X-intercept and Y-intercept values should be both equal the Kd of the antagonist obtained. If the agonist and antagonist are competitive, the Schild plot will have a slope of 1.0 and the X intercept will equal the logarithm of the Kd of the antagonist. If the X-axis of a Schild plot is plotted as log(molar), then minus one times the intercept is called the pA2 (p for logarithm, like pH; A for antagonist; 2 for the dose ratio when the concentration of antagonist equals the pA2). The pA2 (derived from functional experiments) will equal the Kd from binding experiments if antagonist and agonist compete for binding to a single class of receptor sites. Figure 6: (table 2) Results for Schild Plot. From Figure 5 and 6 it is evident that no concentrations of atropine have showed competitive antagonism perfectly. Therefore from the above results it is known that the concentrations of atropine has not shown simple competitive antagonism fairly. Discussion: Reversible competitive antagonism: The binding of drug to a receptor is fully reversible which produces a parallel shift of the dose response curve to the right in the presence of an antagonist. The mechanism of action of acetylcholine at muscuranic receptors: In various gastrointestinal smooth muscles, acetylcholine and its derivatives produce contractions by activating muscuranic receptors. It is generally assumed that the M3 muscuranic receptor plays a key role in mediating this activity. The M3 receptor is coupled preferentially to Gq-type G proteins, resulting in the activation of phospholipase C (PLC) and the formation of ionositiol trisphosphate (IP3) and diacylglycerol (DAG) which are likely to participate in muscuranic receptor-mediated smooth muscle contractions. IP3 causes Ca2+ release from intracellular store and can also mobilize Ca2+ secondarily through Ca2+-sensitive or store-dependent mechanisms. DAG, via activation of protein kinase C, phosphorylates various proteins and can directly activate non selective cationic channels. Figure 7: Diagrammatic representation of calcium and smooth muscle contraction. From the above results the value of shift obtained was 0.378 which denotes the simple competitive antagonism produced by the concentration of atropine used (110-8 M).From the value of shift the pKB value was calculated as 8.4.If atropine has shown simple competitive antagonism then the value of pKB should be equal to 1-X intercept. Therefore pKB=1-X intercept =1-(-8.86) =9.86 We got value of pKB as 8.86.Therefore pKB is not equal to 1-X intercept. Therefore the concentration of atropine (110-8M organ bath concentration) used by our group has not shown simple competitive antagonism effectively. The literature value of pKB is given as approximately 9 and we have obtained the value of pKB as 8.86 which does not fit with literature value. Therefore from the above observations and results i can conclude that a little more high concentration of atropine may serve to produce complete simple competitive antagonism by atropine at acetylcholine muscuranic receptors.

Defense Mechanisms :: Free Essays Online

Defense Mechanisms People use defense mechanisms so often that it is perhaps difficult to pick out individual cases to deal with. Additionally, it would be markedly easier for me to look for evidence of these mechanisms within myself. However, others do display such defenses against anxiety-inducing thoughts, memories, and impulses. In the healthy range of defense mechanisms, repression is key. Simply not thinking about something for a long period of time is often quite helpful. This particular mechanism can at times be rather obvious, as when, in a discussion, a person states that he or she would 'rather not talk about this.' Of course, repression is not always this aware, but in this case it is made manifest by a conscious effort to avoid the topic. Of the neurotic defense mechanisms, humor is perhaps most seen on this campus. Self-deprecating humor helps soften the glare of our shortcomings, especially when they surface in public. Sometimes, jokes are made specific to the situation (I tripped; I'm such a clutz!) but they are often generalized. These jokes are also often not very funny, on the order of "I'm a dumbass...hahaha!" Of the psychotic coping mechanisms, denial is much more obvious than reaction formation. I can think of one specific case, a friend who set his sights too high in sending out transfer applications. As rejections have come in, my notion that he was a non-starter for most of his choices because of grades was proven correct, but this is not something that he can seem to come to terms with. He claims not to understand why myself and several other friends, with near-4.0 GPAs have gotten into several prestigious schools, while he has not. Seems like denial: an inability to face his failings. Reaction formation also interests me a lot, because it is rather counter-intuitive as a defense mechanism. I can't really identify it in others very well, but I can see it in myself. In the case of a couple of failed friendships, in which I felt hurt by the actions of the other person, I compensate for my desire to get closer to them again (which produces anxiety because I am afraid of a repeat) by being very bitter towards them and going out of my way to avoid them.